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The two classes of substrates exhibit different specificity requirements.
We have previously demonstrated two classes of substrates, one comprising large, low-abundance, and difficult-to-assemble substrates that are heavily dependent on SurA and also Skp and FkpA, and the other comprising relatively simple and abundant substrates that are not as dependent on SurA but are strongly dependent on BamB for assembly.
For all classes of substrates, selectivity for a given protease target is controlled by the peptide recognition sequence.
Under such artificial conditions, T3SSs secrete distinct classes of substrates into the extracellular medium, the most abundant of which are effectors and translocators.
In order to establish whether the defective activity of PP2A was observed with other classes of substrates, we tested the activity of immunoprecipitated PP2A towards the small-molecule substrate p-nitrophenylphosphate (pNPP).
Although it is not yet clear in which molecular pathway(s) Dyrk1A is involved, several different classes of substrates have been found to be phosphorylated by this kinase [reviewed in 45].
Similar(44)
In this contribution the general rules and the exceptions in the area of gold catalysis are discussed in order to establish a correlation between the size of the catalytically active element and its reactivity towards different classes of substrate molecules.
An inkling of the cause behind two different classes of substrate came from some outstanding experiments by Jenne (1965).
We hypothesized that fingerprint-based characterization of the biofragment hits (particularly BIO-B6) could be used to provide insights into possible classes of substrate for CYP126 based on previously known TB metabolites.
In contrast to soil dwelling bacteria, where substrate binding proteins for sugars predominate, the predominant functional classes of substrate binding proteins in C. diphtheriae belong to families for amino acid/peptide uptake (e.g., PF00496) and for Fe3+/siderophore uptake (e.g., PF01497), presumably reflecting different nutrient availability in environmental versus host niches.
The preceding sections have demonstrated that neutral genetic drift can lead to substantial changes in P450 activities, and that many of these changes can be understood as resulting from either fairly general increases/decreases in catalytic ability or shifts in preference for different broad classes of substrate structures.
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