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Classes of residue curve maps which give rise to feasible column sequences ae identified.
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In principle, all these drawbacks can be alleviated by introducing additional classes of residues, say partially exposed residues, or exposed residues in contact with other proteins.
Now assume that the protein is subdivided into buried and exposed residues, and that the mutation probability differs among these two classes of residues.
We constructed alleles of S. cerevisiae TRA1 with changes to these different classes of residues (see Fig. 1b).
This is because the unfavorable interaction with the hydrophobic class of side chains is offset by the favorable interaction between sarcosine and the polar and charged side chain classes of residues.
The single residue statistical potential ESOL requires two separate parameter sets, for overall 40 parameters: ei = b or s is the environmental class of residue at position i.
In sharp contrast to what is observed for Tp1 (Fig. 2B C), in which >50% of amino acid residues are extremely conserved (ω = 0), the Tp2 locus does not have a class of residues with an estimated ω = 0 (Fig. 4B C) in either buffalo or cattle parasite sequences.
As a result, only two cysteine sites form the fourth class of residues, which are largely missing from the aligned proteins of the other taxa.
Models M2 (three parameters) and M8 (four parameters) extend M1 (one parameter) and M7 (two parameters) by adding a third class of residues with ω > 1. Model likelihood scores were compared using a Likelihood Ratio Test (LRT) to determine the best-fit model.
As mentioned previously in case of the buried residues, to validate the results obtained in case of the Cα-only structures the sensitivity and specificity values were calculated for two classes of the residues namely interface and non-interface residues.
The rule classified N-terminal residues as stabilizing, or belonging to one of three classes of destabilizing residues: primary, secondary or tertiary.
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