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Exact(25)
This class of binding proteins, both natural and synthetic, has a strong tendency to bind a preformed cleft using a convex binding interface (paratope).
Scatchard analysis predicted for a single class of binding sites with Kd=1.4 nM.
Designed Ankyrin Repeat Proteins (DARPins) represent a novel class of binding molecules.
Antibodies have long been regarded as the only class of binding proteins.
Although many biologically significant interactions involve association of identical subunits, a much larger class of binding events involves interactions of dissimilar partners.
DARPins (designed ankyrin repeat proteins) are a novel class of binding molecules with the potential to overcome limitations of monoclonal antibodies, hence allowing novel therapeutic approaches.
Similar(35)
The thermodynamic parameters showed that the first-class of binding process was primarily driven by entropy and the second-class of binding was driven by enthalpy and entropy.
The Scatchard plot analysis demonstrates that two classes of binding sites are formed with the equilibrium dissociation constants.
A concave upward plot is indicative of nonspecific binding, negative cooperativity, or multiple classes of binding sites.
Scatchard analysis showed that two classes of binding sites existed in the SIN-imprinted polymers, with their dissociation constants estimated to be 7.257×10−5 and 3.828×10−3 mol l−1, respectively.
Therefore, the use of several classes of binding molecules will make suitable crystal formation and the determination of their 3D structure more likely.
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