Exact(16)
Although primarily designed for use with AGO CLASH data, hyb can also be used for the detection and annotation of chimeric reads in other high-throughput sequencing datasets.
Clash data accrued (in mm) was subsequently quantitatively modelled using the probability density function (PDF) and the cumulative distribution function (CDF).
The CLASH data set included a number of previously known interactions (Table S3).
Additional file 2: Excel file containing all tRF-mRNA chimeric reads observed in the CLASH data.
The CLASH data showed that 72 genes were targeted by miR-320b.
Consistent with these results, human AGO1 CLASH data contains thousands of tRF-5 and tRF-3 reads chimeric with mRNAs.
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