In order to clarify the time of Rab21 recruitment to the macropinosomes, we co-expressed GFP-Rab21 with cyan fluorescent protein (CFP) probes specific for phosphoinositides, such as PI 4,5 P2 and PI 3,4,5 P3, in RAW264 cells.
An important target of future research should include clarifying the time course of hospital complications in hospitalized trauma patients, so as to better identify modifiable risk factors.
Because data suggesting an appropriate CDDP injection speed to obtain the best cancer effects is not available, a study clarifying the time dependency of the HCC response to CDDP-HAI should be conducted.
Our findings indicate a need for further clinical studies that include a larger number of patients and a standardized protocol of sampling, in order to clarify the time course of cTnI and cTnT levels after exercise stress in patients with and without CAD.
Because our seismic and modeling results have clarified the time sequence of the tectonic regimes, we can submit a neotectonic synthesis of SWJ under the influence of oblique subduction of an oceanic plate.
To clarify the time course of the brain damage changes, we investigated the histological changes of the same individual with magnetic resonance imaging (MRI) after 5, 9, and 57 weeks of recovery following the HI insult.
However a further exploration is necessary to clarify the time course of expression and to correlate miRNA changes with the severity of stroke.
To clarify the time evolution of the entire higher energy range, we obtained time-resolved PL spectra with time intervals of 0.2 ns, as shown in Fig. 5a.
Future longitudinal research using hair cortisol analysis is warranted to clarify the time course of social mobility in relation to long-term cortisol, to investigate other underlying psychosocial factors implicated in these associations, and to determine the exact health implications of the neuroendocrine perturbations in individuals with limited economic resources.
Further longitudinal investigations with more time points following surgery will clarify the time course of these changes in FA, and these studies are ongoing.
