Sentence examples for clarified sample from inspiring English sources

The reliable analytical performance of the biosensor array was demonstrated by application in a clarified sample of inoculum sludge.

50 µL of each clarified sample was assayed in a 96 well plate by mixture with the appropriate volumes of assay buffer, substrate, and water.

Briefly, the clarified sample was added to 2 mls of Talon™ cobalt resin (Clontech) sand mixed at room temperature for 30 minutes.

The clarified sample was then subjected to buffer exchange using tangential flow filtration with a 5 kDa membrane from Pall Corp. (Ann Arbor, MI).

Before injection into the GC instrument, clarified samples and standards were filtered through 0.45-mm Whatman nylon filter (Whatman, UK) to remove insoluble materials that could block the column.

Clarified samples containing RVF-VLPs were subjected to high-speed ultracentrifugation at 82,000 g for 16 h using either a SW-28 or SW50.1 rotor, depending on sample size.

Supernatants from the clarified samples were used for isolation of RSV by CEC [5] and presence of RSV was detected by indirect immunofluorescence staining with a blend of monoclonal antibodies that detects both group A and group B RSV (Chemicon International).

Clarified samples were incubated with taxol (20 µM) in the presence of GTP (1 mM) and DTT (1 mM) for 45 min at 37°C and were spun at 70 000 g for 30 min at 30°C through a cushion buffer containing 40% glycerol, 20 µM taxol and 1 mM GTP.

The clarified samples were transferred to HPLC vials for LC-MS measurements.

The clarified samples were mixed with 2 ml of Nickel resin (Qiagen, USA) equilibrated in buffer A following the manufacturer's instructions.

The clarified samples were incubated for 80 min at 120 rpm at 37 °C to optimize the extract activity and centrifuged for 15 min at 15 000 × g at 4 °C.