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After preliminary clarification steps, the proteins eluted from Concanavalin A were concentrated and loaded onto size exclusion chromatography.
Protein A Streamline chromatography was successfully used in the purification process yielding a recovery of about 60% and a plantibody SDS PAGE purity of over 90% but unexpectedly, previous clarification steps could not be totally avoided.
In some cases, two clarification steps may be necessary the first to remove large particulates and the second to remove residual lipids, nucleic acids and any remaining cellular debris.
In this study, corrosion behaviors of materials used in diffusion units and equipment used in juice clarification steps and tubes in evaporators at Ankara Sugar Factory were investigated in terms of juice production and juice clarification processes as well as juice medium at evaporation stages.
We have demonstrated that the residual SmP E was consistently removed to <20 ppm during the flocculation and clarification steps (Fig. 2 and Table 3).
Similar(54)
The sodium hydroxide reacts with the aluminous minerals of bauxite to form a saturated solution of sodium aluminate; insoluble impurities, called red mud, remain in suspension and are separated in the clarification step.
This enabled the analysis of the robustness of precipitation as initial clarification step of downstream process of mAb.
For the clarification step, effective design factors taking into account operators' postures were identified via the task analysis.
The absence of most of kinetochore proteins is not surprising, as chromosomes should have sedimented during the extract clarification step.
In beer production, a clarification step is essential to improve beer stability.
The mimic of the light phase process stream from the final clarification step comprised two separate USD steps.
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