Together these studies are beginning to allow clarification of which steps in the process contribute significantly to metastatic inefficiency, and some of the molecular mechanisms responsible.
Usually a biologist will not require a full description of a protocol, and will prefer a much higher level summary, but may require clarification of certain steps.
Because our work proved useful in unexpected ways, we encourage others to consider a scoping review as a constructive method in their broader research process in that it can contribute to the clarification of next steps.
Some of the open tasks are e.g. identification of the adhesin host receptor repertoire, clarification of the first steps of invasion, species specificity, infection of polarized epithelial cells and transfer into in vivo models.
This enabled the analysis of the robustness of precipitation as initial clarification step of downstream process of mAb.
With the recent live-cell clarification of the linked temporal steps that define clathrin-coated structure initiation, growth, invagination and scission (Kaksonen et al., 2003; Taylor et al., 2011), it is clear that a large number of gene products are involved.
Before ChIP, Triton X-100 was added to chromatin samples at a final concentration of 1% and a clarification step of 10′ at 16,360 g at 4°C followed.
Readers then progress through four key steps: (1) clarification of decision and values; (2) decisional needs and support; (3) considering the options; and (4) advising others of one's decision.
As the first step of clarification of the FIV mechanism, it is important to grasp the mechanism of flow fluctuation in the elbow.
This study constitutes an important step towards clarification of lineage commitment and differentiation blockage of glioma cells.
Step 2. Clarification of decision-making procedures ■ Decision-makers and stakeholders need to know and understand a) how decisions will be made; b) on what basis will these decisions be made.
