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The voltage clamp traces in Figure 3A illustrate the persistent inward currents elicited by nicotine (300 nM, 10 min) in male (top) and female (bottom) layer VI neurons.
In figures, voltage-clamp traces represent the averaged waveform of 3 5 consecutive acquisitions.
(A ) Voltage clamp traces recorded at a holding potential of −70 mV from two granule cells.
In figures, voltage-clamp traces represent the average waveform of 3 6 acquisitions.
To separate the phasic EPSCs from the underlying spillover currents we fit a model to the raw voltage clamp traces.
(A ) Voltage clamp traces of EPCs in response to 20 s, 100 Hz stimulation of the motor neuron.
The fitting procedure was as follows: a peak finding algorithm was used to detect the fast EPSCs in the raw voltage clamp traces.
Whole-cell currents were monitored by voltage ramps from −100 to +100 mV and voltage-clamp traces recorded using the protocol shown in Fig 3A.
Current clamp traces were smoothed with a binomial algorithm at 180 550 Hz and action potentials ('spikes') were extracted by applying a negative threshold to the second derivative of the smoothed trace.
The experimentally determined Boltzmann curve then corresponds with y ∞ rather than y ∞, while determination of the rate constants α and β requires detailed analysis of the voltage clamp traces, as carried out by van Ginneken and Giles [ 39].
Unlike the two-state model of Figure 2(a), the three-state model accounts for the sigmoidal onset of I f activation that may be observed in voltage clamp traces.
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