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But lower expression of IDO occurred in IKKα-null mice (Fig. 10) and in the wild-type mice treated with intrarenal delivery of LV-GFP-IKKα shRNA (which can silence IKKα) 2 weeks before pedicle clamping (data not shown).
One problem with these data is that, as noted above, it is well established that isolated mitochondria are contaminated with plasma membrane and thus it is possible that the immunological and patch clamping data were actually detecting the sarcolemmal KCa.
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We then establish a rigorous method for analyzing force-clamp data using order statistics.
Using MEMFIT, sodium currents of motor axons were modeled using the voltage clamp data of Schwarz and colleagues [13].
b1 The parameters of the description of the A-type K+ current were adjusted to fit published voltage clamp data from nucleated membrane patches from SNc dopamine neurons (representative current traces from Fig. 11A3 of [28]).
b2 The parameters of the description of the ERG-type K+ current were adjusted to fit published voltage clamp data (Fig. 1A of [32]) from human channels heterologously expressed in Xenopus oocytes.
A mechanism-based, Hodgkin–Huxley-type modeling approach is proposed that allows connecting the key parameters of experimental voltage-/patch-clamp data directly to the major control values of the model.
The description of the A-type potassium current, (I_{mathrm{K},mathrm{A}} = g_{mathrm{K},mathrm{A}} p (q_{1} /2 + q_{2} /2) (v+90)), was fit to published voltage clamp data (Figs. 1b1 and b2) [28] using two components of inactivation, (q_{1}) and (q_{2}).
The conductance used for these simulations (120 μS/cm2) was chosen to match the amplitude of the currents from the voltage clamp data, obtained with 100 ms steps from a holding potential of −100 mV to 50 mV in increments of 10 mV.
The conductance used for these simulations was chosen to match the amplitude of the currents from the voltage clamp data, obtained with 600 ms steps from a holding potential of −80 mV to −100 to 50 mV in increments of 10 mV.
Previous reports [18], [19], [24], [31], [32] have focused on the match of models to voltage clamp data and current clamp data in control conditions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com