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(D) HBK001 treatment increased the glucose infusion rate (GIR) in the hyperglycemic clamp test.
(B) HBK001 treatment increased first-phase insulin secretion in the hyperglycemic clamp test.
Forty-one subjects completed the clamp test at both time points.
Euglycemic insulin clamp test was performed as described previously with minor modifications.23 All subjects had two fasting blood samples drawn for glucose and insulin measurements.
Patients were discharged after completion of the hyperglycaemic clamp test.
Each subject underwent two experimental protocols, an isoglycemic-hyperinsulinemic clamp test and a hyperglycemic-hyperinsulinemic clamp test, completed 4 8 wk apart (Fig. 1).
Similar(14)
Forty-five clamp tests were performed at each of the two centers.
On the last day of the treatment, hyperglycemic clamp testing was performed to assess islet β-cell function (Fig. 4D).
At the end of the treatment, half of the mice were used for hyperglycemic clamp testing as previously described52, while the other half were decapitated for sample collections.
Clamp testing of the GDA showed normal hepatic artery pulsation.
This approach has been validated in nondiabetic subjects by using euglycemic insulin clamp testing including labeled glucose administration (16).
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