Euglycaemic-hyperinsulinaemic clamps coupled with 2DOG quantification in organs were used to assess in vivo tissue specific glucose transport and utilization stimulated by insulin in congenics and controls.
Hyperinsulinemic-euglycemic clamps coupled with 2-[C]deoxyglucose were employed 36 days post-myocardial infarction (13 weeks of high-fat feeding) to assess systemic insulin sensitivity and insulin-mediated, tissue-specific glucose uptake in the conscious, unrestrained mouse.
The current was recorded by an Axopatch 200B patch clamp amplifier coupled with the Axon DigiData 1322A or Axon DigiData 1440 analog-digital converter (Axon Instruments).
The current trace was recorded using an Axopatch 200B patch clamp amplifier coupled with the Axon DigiData 1322A analog-digital converter (Axon Instruments) or the BLM workstation (Warner Instruments).
For the validation of the developed model an experiment was conducted in two configurations using a simply supported aluminium plate and a clamped plate coupled with a plexiglas box containing a loudspeaker.
We used patch-clamp electrophysiology coupled to electron microscopy and multi-electrode arrays to dissect synaptic transmission of primary SynI KO hippocampal neurons in which the human wild-type and mutant SynI were expressed by lentiviral transduction.
We used patch-clamp electrophysiology coupled to electron microscopy and multi-electrode arrays (MEA) to define the physiological impact of the Q555X mutation at excitatory and inhibitory terminals of primary SynI KO hippocampal neurons in which either wild-type (WT) or mutant human SynI (hSynI) was expressed by lentiviral transduction.
The BRCT-domain protein Rad4TopBP1 facilitates activation of the DNA damage checkpoint in Schizosaccharomyces pombe by physically coupling the Rad9-Rad1-Hus1 clamp, the Rad3ATR -Rad26ATRIP kinase complex, and the Crb253BP1 mediator.
This raises the question of how the T4 and bacterial clamp loaders achieve proper alignment with the clamp, so that the ATP-driven conformational change in the clamp loader can be coupled appropriately to opening of the clamp.
Crystallographic and electron microscopic views of clamp loaders from bacteria, archaebacteria and eukaryotes emphasize their common architecture and have produced models of how ATPbinding might be coupled to clamp opening/loading.
Moreover, multiple genetic interactions between the 9 1 1 clamp and DNA replication-coupled nucleosome assembly factors, including Rtt106, CAF-1 and lysine residues of H3-H4 were found.
