Sentence examples for clamp binding from inspiring English sources

Indeed, upon closer examination, we find a short sequence immediately downstream of the catalytic domain (QTSMAF, residues 182 187) that form a canonical clamp binding motif (Qxx(L/M xF) found in other proteins that bind the clamp such as Pol II, Pol III, Pol IV, and others (Dalrymple et al, 2001).

This region was previously identified as the binding site of another β clamp binding protein, the δ subunit of the γ complex.

(B ) Clamp binding by PolIIIα in the DNA-bound complex.

(A ) Clamp binding by PolIIIα in the DNA-free complex.

We already showed that the mutation of the clamp binding motif in the exonuclease results in a loss of clamp binding.

Hence, we can expect that in the trimeric Pol III clamp exonuclease complex, both binding pockets of the clamp are occupied, one by the internal clamp binding motif of Pol III and one by the exonuclease clamp binding motif.

These data suggest that the enhanced sensitivity observed with these dinB alleles is dependent on the β-clamp binding motif.

In case of BsuL-CTD, the authors suggest- on the basis of docking studies- that this stretch resembles a β-clamp binding motif and might form interactions with the β-clamp.

Thus, derivatives of DinB(F13V), DinB(D103N), and DinB+, as a control, lacking the DinB β-clamp binding motif (347LVLGL351; [44], [44]) were constructed in the same low copy number plasmids (Table S1).

If ΔdinB strains expressing DinB variants lacking the β-clamp binding motif are as sensitive to either MMS or NFZ as ΔdinB, then it could be inferred that the observed enhanced sensitivity is mediated through interactions with the β-clamp, and is consistent with the idea that these DinB variants are localized at the replication fork.

On the basis of homology modeling with β-clamp binding motifs, Pillon et al have suggested that this region might be involved in interactions with the β-clamp although biochemical evidence of a direct interaction between a prokaryotic MutL homolog and β-clamp is still awaited [16].