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Bam HI and Cla I sites were additionally included in the designed PCR-primers for that purpose (Table 2).
CLA: I actually did the CMAs one year.
CLA: I think the technology you're seeing change is just higher quality in video editing.
CLA: I think the Grammys is helping put music back on the map in people's minds.
CLA: I try to make it so that there's an emotional connection with every artist that I work with, otherwise, there's no point in doing it.
Spe I and Cla I were used to clone AtCYN and OsCYN into the pRTL2HA and pRTL2Flag vectors.
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A 680 bp 5′ upstream region (−650/+30) of PSA gene and a Cla I-truncated 1.1 kb MMTV LTR were used as PSA promoter and MMTV promoter.
The genomes of some other Chironomus species contain a short tandem repetitive DNA element that is characterized by a Cla I-restriction site and therefore called "Cla-element" [ 41].
The multiple cloning sites of the vector were changed to Bsr GI- Bsp EI- Spe I- Bgl II- Sal I- Afl II- Spl I- Cla I- Apa I- Sma I/ Xma I- Bam I- Xba I- Bcl I.
DNA fragments flanking the codY gene, that are, the ∼700 bp XbaI-ClaI upstream and the ∼1000 bp ClaI-KpnI downstream fragments, were obtained by PCR amplification from genomic DNA.
The resulting AflII-ClaI fragment has then been inserted between AflII and ClaI sites of the WT IC1 sequence.
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