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The most effective combination was BJ (1 x MIC) and CLA (1 x MIC) against both clinical isolates, with more rapid bacterial killing achieved with the cagA-positive strain (HP6).
The final concentrations of drugs in each tube were the following: (1) control (without drug); (2) BJ (1 x MIC); (3) AMX (1 x MIC); (4) CLA (1 x MIC); (5) MTZ (1 x MIC); (6) BJ (1 x MIC) and AMX (1 x MIC); (7) BJ (1 x MIC) and CLA (1 x MIC); (8) BJ (1 x MIC) and MTZ (1 x MIC).
In the combination assays, the association of BJ (1 x MIC) with the reference antibiotics (AMX, 1 x MIC, CLA, 1 x MIC, and MTZ, 1 x MIC) determined a reduction of > 6log10 for H. pylori ATCC 49503 after 8 h incubation time (Fig. 2b) and for H. pylori ATCC 43504 after 24 h incubation time (Fig. 2a).
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Clarinet (CLA-1), a novel active zone protein required for synaptic vesicle clustering and release.
Compared with the control, milk fat yield was reduced 8% by the CLA-1 treatment and 21% by the CLA-2 treatment, with milk fat content reduced 5 and 18% by the CLA-1 and CLA-2 treatments, respectively.
Several polymorphic variants of the CLA-1 gene have been described, some of which are associated with phenotypic changes in plasma lipoproteins.
Thirty Alpine does were randomly assigned to 1 of 3 groups and fed diets with lipid-encapsulated CLA that provided trans-10, cis-12 CLA at 0 (control), 3 (CLA-1), and 6 g/d (CLA-2).
Milk fat content of trans-10, cis-12 CLA was 0.03, 0.09, and 0.19 g/100 g of fatty acids for the control, CLA-1, and CLA-2 treatments, respectively.
Does were randomly allocated to 3 treatments: A) unsupplemented (control), B) supplemented with 30 g/d of LE-CLA (low dose; CLA-1), and C) supplemented with 60 g/d of LE-CLA (high dose; CLA-2).
The CLA-1 receptor plays a pivotal role in HDL-mediated reverse cholesterol transport by mediating the selective uptake of free cholesterol as well as of native and oxidized cholesteryl esters.
The CLA-1 (CD36 and Lysosomal integral membrane protein-II Analogous-1) gene is the human homologue of the murine SR-B1 (Scavenger Receptor class B type 1) gene.
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