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Both the fat-DN and muscle-DN mice, unlike Mstn−/− mice, still secrete myostatin into circulation (data not shown).
Furthermore, WAT of Fsp27−/− mice isolated from the gonadal region had a dark-red color and abundant blood vessel circulation (data not shown).
However, mass spectrometric analysis of serum from CONV-R mice only identified SAA1, SAA2 and SAA4 in the peripheral circulation (data not shown) which is consistent with a recent publication [25].
The ectopic expression of human NRP-1 in zebrafish efficiently blocked zebrafish NRP-1 knockdown-induced EC apoptosis, and the zebrafish showed normal vessels with established blood circulation (data not shown), and a normal EC phenotype (Figure 1e, Figure S1, c, d).
Data from sequencing additional isolates from the archival collection of BPI3V indicates that BPI3Va are now only a minority and that members of all three genotypes and their subgenotypes are in circulation (data not shown).
Administration of anti-CD25 mAb results in >90% depletion of CD25+CD4+ cells at the time of transplantation (day 0, Fig. 1B) and at this time point is cleared from the circulation (data not shown), thus depleting CD25+ Treg without impairing effector T-cell responses driven by the graft.
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During two RSV peak circulation periods, both group A and B RSV viruses circulated (data not shown).
Indeed, in 96 hpf ofd1 MO-injected embryos, which had developed severe generalized oedema, circulation was absent (data not shown).
Additionally, sequencing of other BPI3V isolates in the archival collection revealed that TX81-like strains are also found in circulation in domestic cattle herds (data not shown).
Moreover, no significant differences could be observed for IL-23 in circulation or locally in the joints (data not shown).
Quantum dot fluorescence diminished very rapidly, and by 2 h, QD fluorescence in circulation was undetectable by fluorescence imaging (data not shown).
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