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Twenty-three percent of mice that received transplants showed circulating donor cells in the blood, and 7%to14%4% showed donor cells in the tissues.
Bone marrow appears to be the site where donor cells reside long-term, supplying peripheral blood with circulating donor hematopoietic cells that can maintain tolerance.
This is consistent with the appearance of circulating donor cells at 3 4 weeks post transplantation as evidenced by the occurrence of chimerism in CD45.2+ controls transplanted with CD45.1+ Lin− Sca-1+ HSCs (Fig. 2B and data not shown) [22].
This is consistent with the appearance of circulating donor cells at 3 4 weeks post transplantation as evidenced by the occurrence of chimerism in CD45.2+ controls transplanted with CD45.1+ HSCs (data not shown).
Chimeric animals with <80% of circulating donor cells were excluded from the analysis.
We previously conducted a study of skin epithelial tumours in skin cancers developing after kidney transplantation, a situation similar to pregnancy because it is characterized by the presence of a small number of circulating donor cells [ 15].
Similar(53)
Circulating donor-reactive effector memory T cells have been associated with allograft injury in humans.
Immunologic factors linked to IF/TA are acute, sub-clinical and chronic rejection, HLA mismatching and circulating donor-specific anti-HLA antibodies[7], [8].
Presence of circulating donor-specific antibodies (DSA) has been recognized as a relevant cause of allograft rejection [ 37, 38].
PTC C4d deposition has been shown to be correlated with circulating donor-specific antibodies, that induce endothelial cellular apoptosis independent of the action of the complement system [ 19].
Levels of chimerism achieved 8 months after BMT did not differ between young transplant recipients (circulating leukocytes donor-derived: 95.0%; SEM = 1.9; N = 5) or old recipients (circulating leukocytes donor-derived: 97.6%; SEM = 0.28; N = 4).
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