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Two other peptide hormones released from the corpora cardiaca of Drosophila, DILPs and AKH, have clear roles in regulation of circulating carbohydrate levels and in responses to starvation and stress [2], [3], [26], [27], [47], [58], [59].
The conservation of regulatory endocrine mechanisms controlling circulating glucose levels in Drosophila larvae is further evidenced by the presence of cells in the corpus cardiacum that produce and secrete adipokinetic hormone (AKH), which functions in glucose homeostasis by mobilizing stored energy reserves and raising circulating carbohydrate levels [ 8].
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We previously showed that mNSC-ablated flies exhibited generally higher levels of stored energy (trehalose, glycogen and lipid) and an altered profile of adult, fasting, circulating carbohydrates compared with controls [14].
PPARγ activation promotes adipocyte differentiation and storage of excess circulating carbohydrates as triacylglyceride [ 28].
In order to prevent this progression, current research focuses on strategies to protect beta cells against the toxic microenvironment produced by circulating carbohydrates and lipids.
As Atf4 is expressed throughout fly development (Hewes et al. 2000), it may regulate glycolysis in other situations as well: notably, Atf4 mutant flies are lean and have reduced circulating carbohydrates, suggesting a role in metabolism (Seo et al. 2009).
Surprisingly, the levels of circulating carbohydrates (trehalose and glucose) and triacylglycerides (TAGs) were not modified in DILP6 mutant larvae at the wandering stage, suggesting that DILP6 function, although limiting for growth control, is dispensable for metabolic regulation.
The significant elevation in circulating sugar levels suggests an inability to take up and store carbohydrate.
The effect of elevated circulating sugar levels following IPC ablation in immature flies is reminiscent of a mammalian diabetic phenotype and supports the conserved role of DILPs in carbohydrate homeostasis.
Therefore reduced circulating leptin after carbohydrate ingestion may be an important factor in controlling body weight in slim adults.
L-selectin on the circulating lymphocytes binds carbohydrate epitopes of PNAd, facilitating homing of these naïve lymphocytes [ 37].
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