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In addition, expression of HvCCA1 was analyzed in circadian samples of the barley cultivars Azumamugi, Hayachinemugi, Kaikei 84, and Yukiwarimugi.
For circadian samples, plants were grown in SD (8 h light/16 h dark) or LD (16 h light/8 h dark) conditions.
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Gene expression was analyzed in leaf samples harvested from Bowman, Bowman(Ppd-H1), Bowman eam5), and Bowman(Ppd-H1 + eam5) grown under LD and SD conditions, from a developmental series of leaf samples from Bowman and Bowman eam5) and from diurnal and circadian sampling of Bowman and Bowman eam5) leaves.
To avoid blood composition changes promoted by diet and circadian rhythms, samples were always collected in the early hours in the morning and after a fasting period of 8 hours.
Because of the circadian rhythm sampling results are also influenced by sampling time [ 12].
25 The lack of consensus from these studies can be ascribed to different definitions of circadian rhythm, sampling times and methods of data analysis.
In order to analyze corticosterone secretion during the circadian cycle, plasma samples were collected 1 hour after lights on and 1 hour after lights off.
An E. pulchra head cDNA library from circadian and tidal samples was also constructed and used to isolate the full-length Eurydice period (Epper).
To evaluate the corticosterone circadian rhythm, plasma samples for corticosterone measurements were collected at the circadian nadir (morning, 1 h after lights on) and circadian peak (evening, 1 h before lights out).
To evaluate the corticosterone circadian rhythm, urine samples for corticosterone measurements were collected at the circadian nadir (morning, 1 h after lights on) and circadian peak (evening, 1 h before lights out).
The aim of the present review was to provide a brief, yet comprehensive, overview of current data examining sleep characteristics (ie, quantity, quality, and circadian changes) in samples that included patients 80 years or older, who were admitted to the intensive care unit (ICU).
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