CIM tests the significance of candidate QTL by combining IM with multiple regression analysis.
CIM tests were performed at 1-cM steps, with a 10-cM window size.
When testing a putative QTL, CIM includes additional markers as covariates in the model to help control for effects of other QTL.
NOTE: CIM-Inbound feed was run manually during testing for immediate results and validation.
Since it has been used in the literature on simulated and real CIM results [21], we also tested the bootstrap CI method implemented in QTL Cartographer, which lacks the background marker selection step after each new bootstrap data set is created [18].
We have observed that increasing the level of detail in a data set, which is often an interim goal for us as experimentalists, weakens the performance of the most widely used confidence measure, which had heretofore not been explicitly tested for its performance with CIM results.
Pragmatically, measurements of peroneal CMAP could be implemented to diagnose all patients with ICU-acquired neuromuscular disorders, either CIP or CIM, while other tests, such as measurement of dmCMAP, could be used to differentiate CIM from CIP.
For each trait, we used our CIM QTL peaks as the initial positions in MIM, tested to determine whether all remained in the model simultaneously, and omitted any that did not.
Furthermore, we tested the cell migration and invasion ability using CIM-Plate precoated with Matrigel or not on xCELLigence system.
DOI: http://dx.doi.org/10.7554/eLife.01298.018 Resistance of CIM mice to the type I virulent RH-YFP strain in vivo was exploited to test linkage of resistance to the IRG system in a (CIM×BL/6 F1×BL/6 backcross.
