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In situ hybridization against the EGFP mRNA showed a similar chronological expression pattern as the endogenous PK2 mRNA, reflecting the activity of the PK2 promoter used for the transgene approach (data not shown).
The benefit of finding a genetic association with specific genes is that we can begin to understand more about how the parasite triggers the development of ocular disease during embryogenesis and fetal development by examining what is known about the chronological expression and localization of COL2A1 and ABCA4 during development and after birth.
Testosterone and FSH impact a common chromatin remodelling mechanism during spermiogenesis through chronological expression of different molecules required for this biological process to take place.
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With a mechanism regulated by endogenous cell machinery, this nanocomplex realized the successive stimulating motif release and the dynamic imaging of chronological mRNA expression during neural stem cell differentiation without the use of transgenetic manipulation.
To test whether transcriptional changes of the 27 identified modules associate with chronological age, an expression profile for each module was constructed by determining the mean expression of the genes within a detected coexpressed PPI module per individual.
Less is known about the chronological pattern of expression of ABCA4 in the eye during development compared to COL2A1, although a high frequency of cDNA clones positive for ABCA4 has been reported in screens of cDNA libraries prepared from developing mouse eye [38].
Special attention is paid to the chronological analyses of embryogenetic expression of brainstem function and to the spatial patterning of the functional organization/architecture of the brainstem nuclei.
Consequently, in subsequent experiments, we measured the effects of ectopic CLN3 expression on chronological lifespan under conditions that avoided adaptive regrowth by periodically reseeding cells in medium that had been pre-depleted of nutrients.
It is worth noting here that the difference in age predicted by global gene expression and chronological age for a given sample may not simply be due to error in prediction methodology.
Differentiation of fibroblasts into adipose tissue requires chronological changes in the expression of numerous genes [ 12- 14].
The cross-sectional nature of the investigation also limits inferences for the associations of gene expression with chronological age.
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