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We induced hypothyroidism, followed by supplementation with 3,5-diiodothyronine (T2) or 3,5,3′-triiodothyronine (T3) in zebrafish exposed to different chronic temperatures.
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We first measured gene expression responses in the embryos by RNA-seq to characterize molecular signatures of mild, chronic temperature stress in an unbiased manner.
Additionally, southern California populations exposed to chronic temperature stress survived longer than populations from northern California.
(2011) study were less reproductively mature than the fish used in the present study and were subjected to fluctuating acute temperatures rather than chronic temperature exposures.
Thus, for example, cholesterol synthase and HMG-CoA reductase were downregulated as a result of chronic temperature elevation, as previously reported by Podrabsky and Somero [ 30].
Based on these findings and the same experimental fish, the current study aimed to further understand how the chronic temperature elevation affected molecular processes at the levels of gene and protein expression in the heart, as a key organ for thermal plasticity and acclimation in salmonids [ 31, 32].
In this work we compare the transcriptional responses in Atlantic salmon exposed to chronic high temperature (19°C) and low oxygen saturation (4-5 mg/L) stress.
Two particular HSPs previously found to be up-regulated in fish exposed to chronic high temperature are Hsp70 and Hsp90 [ 45].
In this work we wanted to compare the transcriptional responses to chronic high temperature and low oxygen stress in Atlantic salmon to elucidate possible negative effects on farmed Atlantic salmon as a consequence of climate change.
This suggests that although chronic high temperature and low oxygen stress affects many identical mechanism in fish, they do not necessary invoke these effects through differential regulation of the same individual genes.
The upper limit of chronic high temperature exposure for all lines tested here was about 34°C, where plants continue to grow and flower but exhibit reduced seed yield (data not shown), consistent with the idea that some trienoic fatty acids are essential.
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