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Following hybridization, chromosomes were washed with 0.2×SSC, counterstained with YOYO-1 (Sigma) and mounted in DABCO (Sigma) antifade solution.
Chromosomes were washed three times with PBS, stained with propidium iodide (1 µg/ml in PBS) for 10 minutes, washed again and covered with mounting medium (AF-1, Citifluor) and a cover slip.
After hybridisation, chromosomes were washed with 50% formamide/2 × SSC and 2 × SSC at 42°C.
After hybridization, chromosomes were washed in 0.2× SSC and counterstained with YOYO-1 and mounted in DABCO antifade solution (Sigma) [ 19].
Then the chromosomes were washed in 0.2XSSC (Saline-Sodium Citrate: 0.03 M Sodium Chloride, 0.003 M Sodium Citrate), counterstained with YOYO-1, and mounted in DABCO.
Then the chromosomes were washed in 0.2 × SSC (Saline-Sodium Citrate: 0.03 M Sodium Chloride, 0.003 M Sodium Citrate), counterstained with YOYO-1, and mounted in DABCO.
Similar(53)
The chromosome preparations were washed in 0.2XSSC, counterstained with YOYO-1, and mounted in DABCO.
The chromosome slides were washed as follows: 4X SSC for 5 min at room temperature, 0.1% IGEPAL/4X SSC for 5 min at room temperature, and 4X SSC for 5 min at room temperature.
The chromosome slides were washed using the following series; 4X SSC for 10 min at room temperature, 0.1% IGEPAL/ 4X SSC for 10 min at room temperature, and 4X SSC for 10 min at room temperature.
SpectraVision™ probes were hybridised to the chromosome preparations for 48 h, then slides were washed and counterstained with DAPI.
Prior to RNA extraction, larvae and pupae were washed twice in DEPC-treated water and larvae sexed by PCR using Y chromosome-specific primers [ 59].
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