The purified chromosomes were embedded in agarose miniplugs to achieve approximate concentrations of 1 million chromosomes per 40 μl volume before being treated with proteinase K as described previously61.
The first batch of 5.1 × 106 chromosomes were embedded in 30 agarose miniplugs, each containing 1 × 105 or 2 × 105 chromosomes, and used for construction of a HindIII library.
Flow-sorted chromosomes were embedded in agarose miniplugs and used to construct 5DS-specific BAC library as described by Šimková et al. [ 48].
Neuronal loss in these patients is expected to be due to the abnormal function of a protein encoded by the TIMM8A gene on the X chromosome and embedded in the inner mitochondrial membrane.
These processes depend inextricably on the dynamics of the cytoskeleton, endomembrane system, and nuclear envelope, on the structure of chromosomes, and are embedded in the complex eukaryote cell cycle [ 10- 13].
EBV plasmids are found in association with chromosomes early in prophase and embedded in mitotic chromosomes detected by staining for cohesin consistent with this hypothesis (Supplementary Figures 4 and 6); (4) when the sister chromatids separate at anaphase, the tethered EBV sister plasmids do so too.
Human chromosome centromeres are embedded in several thousand kilobase pairs of repetitive alpha satellite DNA consisting of diverged tandemly repeated 171 bp monomer units [24].
Ferris and Goodenough (1994) showed that the two alleles on chromosome 6 are embedded in a region of DNA that contain translocations, inversions, duplications, and deletions, which creates a region that is suppressed for recombination.
In What Is Life? (1944), one of the fundamental questions the physicist Erwin Schrödinger posed was whether there was some sort of "hereditary code-script" embedded in chromosomes.
The artificial chromosome generating mechanism is embedded in simple genetic algorithm (SGA) and the non-dominated sorting genetic algorithm (NSGA-II) to solve single-objective and multi-objective flowshop-scheduling problems, respectively.
