In order to evaluate the qualitative distribution of mapped sequences along bovine chromosomes, a chromosomal mapping of the 69,136 putative methylated regions was generated and plotted alongside genomic coverage data for genes and CpG islands as well as a cytoband mapping adapted from Liao et al. [ 38].
A point mutation was discovered, after chromosomal mapping to chromosome 6, in the mir-96 seed region (+15 A > T), a region fully conserved between species.
Chromosomal mapping was verified on metaphase chromosomes using the following combination of probes: F9 and WCP22 (Fig. 2a), A10 and WCP22 (Fig. 2b), F9 and L9 (Fig. 2c), F9 and B22 (Fig. 2d), L9 and K3 (Fig. 2e), B22 and L21 (Fig. 2f).
By chromosomal mapping, FATE/BJ-HCC-2 gene was located to chromosome Xq28, where a lot of important CT antigen encoding genes are located, such as MAGE family and NY-ESO-1 (De Plaen et al, 1994; Chen et al, 1997a).
Chromosomal mapping has shown that L. lactis has a circular chromosome (fig. 3 A ) with six ribosomal operons that are transcribed divergently from the origin of chromosomal replication (Davidson et al. 1996).
Further, comparative chromosomal mapping studies have shown that duplicated zebrafish genes often reside on distinct chromosomes that exhibit extensive blocs of conserved synteny with their mammalian counterpart [ 39, 40].
Koike, M. et al. C/EBP-ɛ: chromosomal mapping and mutational analysis of the gene in leukemia and preleukemia.
We report here the molecular cloning and chromosomal mapping of three loci from the Drosophila genome that contain such sequences.
Pshezhetsky, A. V. et al. Cloning, expression and chromosomal mapping of human lysosomal sialidase and characterization of mutations in sialidosis.
Stibitz, S. & Yang, M. S. Genomic fluidity of Bordetella pertussis assessed by a new method for chromosomal mapping.
Fiore, A. E., Michalski, J. M., Russell, R. G., Sears, C. L. & Kaper, J. B. Cloning, characterization, and chromosomal mapping of a phospholipase (lecithinase) produced by Vibrio cholerae.
