Sentence examples for chromatography section from inspiring English sources
The plates were developed using above-mentioned mobile phases (in thin layer chromatography section) and scanned.
sphaeroides reaction centres that were modified with a poly-histidine tag on the C-terminus of the M-polypeptide, and purified by nickel affinity chromatography (see Section 2).
After biotinylation and solubilization (~40% yield), recombinant IP3R1 was purified using streptavidin and then cleavage by PreScission protease (~50% yield), anion-exchange (~16% yield) and size-exclusion (~70% yield) chromatography (see the Experimental section).
Selected purified proteins were also spun to remove aggregates, analysed by analytical size exclusion chromatography (SEC) (see Methods section) and the calculated yields are shown in the last column headed "analytical SEC" in Table 2. Single peak integration was linear in the range of 40 – 700 ng using maltose binding protein (MBP) and bovine IgG as standards (data not shown).
The protease was purified from soluble dialysate by gel filtration chromatography as described under methods section.
To identify lipids present in AlbuMAX, we extracted them using organic solvents and analyzed them by high performance liquid chromatography (see Materials and Methods section).
Recombinant PADRE-BAFF was purified by using a His-tag affinity chromatography column as described in Section 2).
Additional enhanced performance observed from the chromatography setup is expanded in section S2 in the Supporting Information.
Furthermore, analysis of the microdialysates for the drug VGB itself, as well as GABA, a downstream marker, can be performed simultaneously by the same high-performance liquid chromatography (HPLC) method (see Methods section).
The recombinant protein was purified to homogeneity by immobilized metal affinity and gel-filtration chromatography as described in the Experimental section and analysed by SDS/PAGE.
For this purpose, tetrapyrroles were extracted from the various E. coli strains and separated using reversed phase chromatography as described in the Experimental section.
