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The fabrication of proposed CBA-g-PVA HNFM offers a feasible alternative for construction of ion-exchange chromatograph column for bio-separation and purification engineering.
"We have presented the first demonstration that a monolithic chromatograph column can be used to separate small molecules on a timescale that is compatible with NMR/MRI detection, an important first step to portable chromatographic devices," says Vikram Bajaj, a project scientist in the Pines' group who is the corresponding author of a paper describing this work in the journal Analytical Chemistry.
In this latest development, he led a collaboration in which a remote detection NMR/MRI technique that can rapidly identify the chemical constituents of samples in microfluidic "lab-on-a-chip" devices was used to perform analyses in a microscale monolithic chromatograph column.
This work describes the design, computational prototyping, fabrication, and characterization of a microfabricated thermal conductivity detector (μTCD) to analyze the effluent from a micro-gas chromatograph column (μGC) and to complement the detection efficacy of a surface acoustic wave detector in the micro-ChemLab™ system.
Fraction B (5 g) was further subjected to RP-18 chromatograph column, eluting with MeOH-H2O (40 60, 60 40, 80 20, and 100 0) to afford five fractions: B1 B5.
All GC-MS runs were conducted on a Hewlett-Packard Series II Plus gas chromatograph (column: 30 m × 0.25 mm HP-5MS Hewlett-Packard Co). coupled with a Hewlett-Packard 5989B mass spectrometer.
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The measurements were performed by using carrier gas argon and chromatograph columns filled with molecular sieve NaX.
The initial platform called the VLSS for very large scale synthesizer utilized large Pharmacia liquid chromatograph columns as reactors and could synthesize up to 75 millimoles of material.
GC MS was carried out using the gas chromatograph and column previously described, with identical operating conditions, coupled to an ISQ mass spectrometer (Thermo Scientific).
Gas samples were taken using tight syringes and injected into the gas chromatograph's column.
Gas samples were taken by tight syringes and injected into gas chromatograph's column.
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