Afterwards, all chromatogram contigs obtained were manually edited removing ambiguous chromatogram areas, which resulted in the nearly full-length 16S bacterial rDNA sequences of about 1500 bp.
Therefore, we also calculated the extract ion chromatogram areas for all corresponding noncitrullinated peptides.
The relative quantification of metabolite features was based on EIC (extracted ion chromatogram) areas.
Additionally, according to the analysis of chromatograms area it may be stated that supplementation of bread with broccoli sprouts significantly influences protein digestibility.
This agrees in a 98.86 % with the conversion values obtained from the chiral HPLC chromatograms areas, where the conversion determined was 47.54 %.
Total DPPH chromatogram peak area was equal to 100%% and identified compounds area respectively was calculated using proportion.
Each peak was quantified based on chromatogram peak area, and a linear best-fit equation was applied to correlate peak area and molar concentration.
In each stage, an 8-test design was used in which a high and low limit was set for each variable, and the chromatogram peak area of the derived amino-NBD was measured.
The registered chromatogram peak area at the selected wavelength (210, 254 and 280 nm) corresponds to the relative quantity of DOM in a specific molecular size fraction that is not determined in the present study.
The standard calibration curves for known amounts of ATP, ranging from 0.025 to 10.0 µmol/L, were linear (R>0.999) and could be described by the linear regression equation: y = 0.4992*x−0.0463 (n = 4, P<0.0001, r = 0.9997), in which y is the ATP concentration in micromoles and x is the chromatogram peak area.
The HPAEC chromatogram peak area change for xylose is shown.
