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The two concentrations were chosen according to previous study, in which chose concentrations of AHLs from 10too 2 μM (Mathesius et al. 2003).
We chose concentrations of 0, 0.25 0.25, and 0.5 µg/ml CdCl2 and performed RT-PCR at 0, 6, and 24 h on KO and Inv2 cell populations.
For the samples incubated with fatty acids, based on ThT and immunoblot results, we chose concentrations, near and above the respective CMCs, to examine by CD.
We chose concentrations of agonist in the lower portion of the dose-response dynamic ranges for AaOR2 (Fig. S1) and AaOR8 [33] in order to minimize signal desensitization, which tends to increase at higher concentrations.
Third, we induced microtubule disruption in U2OS cells after performing a nocodazole titration up to 20 µM; we chose concentrations low enough to avoid complete cell withdrawal and cell rounding, while allowing preservation of cell morphology (data not shown).
Based on the results of this preliminary experiment, we chose concentrations of 8 pg/µl and 200 pg/µl to generate, respectively, the MspI- and MseI- male and female Representations.
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We chose concentration ranges of mercury according to Monnet-Tschudi et al. (1996), who found a concentration of 1 μM to be cytotoxic.
Students can choose concentrations in entrepreneurship, finance, management information systems and marketing.
After that, the samples were evacuated and then saturated to 100 % with brines of chosen concentrations.
It is worth to mention that MSNs are frequently studied in vitro at concentrations up to 100 μg/mL, so our chosen concentrations are fairly low.
Empirically, it was determined that at the chosen concentrations for this study, aluminum, iron, phosphorous, potassium, and sodium all have negative impacts on fracturing fluid stability.
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