To address this question, we chose a mouse knockout lacking ABCD1, a peroxisomal transporter of very long-chain fatty acids (VLCFA).
In this report, we chose a mouse model of spontaneous breast cancer to investigate the function of Usp18 in solid tumour development in vivo.
As a model system, we chose a mouse fibroblast line for which biophysical data included cell speed, membrane protrusion activity, cell spread area, surface adhesion, and membrane retraction (previously measured by Maheshwari et al [ 10]).
To study the contribution of LSCs or other nonhepatocytes to new hepatocytes, we chose a mouse model of chronic liver injury caused by a choline-deficient, ethionine-supplemented (CDE) diet.
We chose a mouse ESC clone that allows the efficient insertion of DOX-inducible transgenes by Cre/loxP-mediated cassette exchange (Iacovino et al., 2011) and equipped it with an additional MN-specific reporter transgene that allows magnetic enrichment of this cell type (Fig. 3A).
If you have a Roborovski hamster, choose a mouse cage over a metallic hamster cage because the young ones can squeeze between the bars and escape.
Since the xenograft models utilize immune deficient mouse strains to avoid graft rejection, immunologic microenvironments of transplanted GBM cells can be specifically modified by choosing a recipient mouse strain with defined immune deficiency status.
Of the available arsenic rodent models (Table 1), we chose a sensitive mouse model to study the mechanisms, that is, female C3H mouse, primarily because of positive carcinogenicity data in a gestational arsenic exposure from the Waalkes group.
We chose a SCID mouse as described earlier, because it was difficult to prepare RCC4 tumour xenografts in nude mice (Ogura et al, 2005).
To determine whether we could detect even rarer events, we applied 3D to mtDNA isolated from muscle samples of mice, choosing a site encompassing the light chain origin of replication (Supplementary Note 4).
