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Under optimized experimental conditions mentioned above, some species can possibly interfere with cholesterol determination.
Cholesterol determination.
The full procedure of cholesterol determination was followed according to the instructions of the kit provided by Biosystems, Barcelona, Spain.
Part I was dissolved in 1 mL of HEPES buffer for total protein analysis and Part II was dissolved in 1 mL of dehydrated alcohol for cholesterol determination.
The method of LDL cholesterol determination used at screening was used throughout the study for an individual subject, regardless of changes in triglycerides.
The remaining liquid yolk was subjected to total cholesterol extraction immediately following the procedure for cholesterol determination as described by Choe et al. [ 19].
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The results are highly correlated with LDL cholesterol determinations by ultracentrifugation (r>0.95) and dextran sulfate precipitation (r>0.95), but an increased recovery of small, high density LDL particles is observed, which more adequately reflects the atherogenic risk of LDL.
Variability in cholesterol measurements frequently renders the interpretation of single or repeated cholesterol determinations problematic, complicating employee counseling within screening programs.
At E18.5, fetal blood was collected after decapitation and blood pooled from 3 fetuses was used for cholesterol determinations.
Lipases can be immobilized on the sensing surface and can function as lipid biosensors for blood cholesterol determinations [ 106].
Plasma glucose, triglyceride, total cholesterol, LDL cholesterol, and HDL cholesterol determinations were done using a biochemical autoanalyzer (AU-400; Beckman Coulter, Fullerton, CA, USA).
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