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Our data provide both mechanistic insights and quantitative readouts of vector-mediated genotoxicity, allowing a relative ranking of different vectors according to these features, and inform current and future choices of vector design with increasing biosafety.
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The simplified TALE assembly combined with multiple choices of vectors will facilitate the broad use of TALE technology.
To adapt the methods to study other genes, modifications of restriction sites, PCR conditions, choices of vectors, or other experimental parameters may be required.
Facilitating this process is the choice of vector, which is often situationally-specific and limited in engineering capacity.
Our findings also reinforce the many years of work suggesting the tf-idf representation is a strong choice of vector space, but only for longer units of text.
Delivery of the oligonucleotides, choice of vector, chemical modification to stabilize against nucleases and avoidance of side effects (e.g. stimulation of interferons) are major challenges.
The unique characters of NDV lying in its broad host spectrum and ease in production using embryonated chicken egg make it a choice of vector for expressing foreign gene.
However, the complexity can be improved even further in practice with a clever choice of vector space.
This variant will help us figure out the appropriateness of our choice of vector quantization to determine the starting point of our clusters in the feature space.
When m is large and m n ′≈n, the number of variables is decreased by a factor 2 if we use our special choice of vector space instead of a random one.
The choice of vector permitted imaging of HNF6 (cloned into the MCS) by fluorescence microscopy.
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