In these cases, and contrary to X-inactivation, ASE was not stable within a clone lineage, as the allele that was expressed could alternate, and the choice of expression was made independently for each gene, not for a chromosome in its entirety.
Clearly, making a rational choice of expression system to use for the characterization and study of cloned xenobiotic transporters is a critical part of study design.
It is concluded that there is no unique host system that can consistently yield high expression levels for a wide range of single chain antibodies; instead, product sequence and end application often dictate the optimum choice of expression host.
Special consideration has to be given to the choice of expression system, the design of the expression vectors, and the purification strategy to obtain a pure product free from toxins and contamination.
Selected targets can be optimised through construct design (e.g. to minimise protein disorder), switching to a homologous protein, and selection of experimental methodology (e.g. choice of expression system) to prime for efficient progress through the structural proteomics pipeline.
Here, we compared the impact of different parameters such as the choice of expression hosts as well as metal affinity columns in order to identify the most effective approach in obtaining highly pure recombinant cationic biopolymers with acceptable yield.
Conclusions: This work offers a timely guide for metabolic disease investigators to enable informed end-user decisions regarding choice of expression microarray platform best-suited to specific research project goals.
But that means that an optimal choice of expression for a speaker who wants to communicate that the actual world is w3 would be 'three', because this maximizes the chance that the literal interpreter selects w3.
Recently, a Brainbow transgenic strategy has been developed [13], in which Cre/lox recombination was utilized to create a stochastic choice of expression among three different fluorescence proteins.
