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Our conclusions hold for all choices of alignment and threshold; here we only present the results based on mammalian conservation with PhastCons score threshold of 0.5.
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The choice of alignment tool for each biologist should be tailored to the goals of the sequencing project.
A quick comparison of the scatterplots for these three datasets shows that the choice of alignment method has little affect on the resulting accuracy and reproducibility for any of the methods.
Likewise, the observed limits of length variation, specific for each domain superfamily would be particularly relevant in the choice of alignment length search filters commonly applied in protein sequence analysis.
Although sometime it is desired to have different parameters for different applications, the real-valued gap opening and extension scores as well as the choice of alignment methods make it hard to optimize these parameter settings for a certain task.
We examined the effect of choice of alignment target on the identification of eQTL.
We highlight that modeling errors will be influenced by parameterization and choice of alignment software.
Hence, to some unknown degree, our analysis is necessarily biased by the choice of alignment.
The choice of alignment program is a major consideration in localizing sequences on the genome.
The number of variable and informative sites is indicated under "#sites" and varies depending on the choice of alignment.
Overall, the discrepancy between different alignments confirmed that the choice of alignment algorithm has a strong impact on estimates of positive selection [ 46].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com