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These metrics enable the development of a method that identifies efficient selection choices for sequencing.
Here, we introduce a statistical framework that enables systematic comparison among subject-selection choices for sequencing.
Hence, solely employing the Lambert solver to rank transfer legs could lead to incorrect choices for sequencing of multi-target trajectories.
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The high coverage afforded by Illumina sequencing has made it an increasingly popular choice for sequencing microbial communities.
For decades, mass spectrometry has been the tool of choice for sequencing the oligosaccharide components of purified glycoproteins.
Additional file 2: Figure S4 illustrates the detection overlap between DESeq and edgeR for two contrasting choices of sequencing depths and n-biological replicates: n = 2 at 100% depth and n = 4 at 25% depth.
This is now the method of choice for sequencing of most other genomes, including human (as performed by Celera Genomics).
The absence of VRN2 from Brachypodium was an unexpected finding but this may reflect the choice of accession used for sequencing.
The latter appears to have emerged as the de facto choice to better control for sequencing errors [ 3, 13].
In total, our approach provides quantitative guidelines for the choice of sequencing depth necessary to achieve a desired level of analytical resolution.
The visual determination of the position and context of each new mutation strongly facilitates the choice of sequencing primers for the validation of the mutation.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com