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Several aspects, including animal care, sampling times and choice of tissues, as well as recommended endpoints and methodologies are addressed and, for each aspect, a standard procedure is proposed.
Revisiting these data has the potential to provide new insights into mRNA processing under multiple conditions, while also guiding the choice of tissues and conditions for new investigations.
This controlled for any bias in GO categories arising from the selection of genes on the chip and the choice of tissues or cell types examined.
While this choice of tissues is somewhat arbitrary, these tissues have undeniable physiologic importance.
Second, the degree of specificity revealed depends on the number and choice of tissues included in a study.
The choice of tissues was deliberately heterogeneous to check the ability of MMDiff to identify both subtle changes (as expected between cortex and cerebellum) and more marked changes between brain tissues and liver.
Similar(53)
Soft tissue reconstructions of extinct animals inevitably contain a high-degree of subjective estimation [55], in this case in the geometry and structure of body segment volumes and airs sacs, in addition to the choice of tissue density values.
Interestingly, the choice of tissue type also seemed to be influential.
These discrepancies might result from differences in the choice of tissue samples.
Only hepatic microarray data was available in the literature, which therefore also contributed to our choice of tissue.
Related to the tissue and cell specificity of epigenetic profiles is the appropriate choice of tissue for the disease of interest.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com