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Considerations for designing the in vitro surveillance database to support the development of antibacterial agents include the choice of pathogens to study, the sample size of isolate collections needed, and target patient populations from which clinical isolates should be collected.
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Our study is also limited in its choice of pathogen: we focussed on S. aureus due to its relatively high prevalence and impact on public health but skin infections can also be caused by a Streptococcus bacteria which may have other AMR patterns and would be relevant to also consider in treatment guidelines.
Despite newer molecular techniques being applied in diagnostic microbiology, recent analyses confirm the use of automated blood culture systems as the primary choice for detection of pathogens from blood specimens [ 1, 2].
Perhaps a crucial asset for detection of ongoing DNA transfer and its vertical inheritance is the choice of an intracellular pathogen persisting in the host throughout its lifetime, providing the epidemiological requirements for a LDT population genetics study.
This choice of bacterial and viral pathogens covered a wide variety of mechanism to invade host cells.
The choice of this clinically important pathogen for fundamental and applied genomic studies is prompted by the availability of several fully or partially sequenced strain genomes [ 16- 18].
Our study supports the choice of antimicrobial treatment covering atypical pathogens (e.g. macrolides) for all patients with CAP who are admitted to hospital after prior treatment with beta-lactam antibiotics.
We need to know the current status of pathogens and antibiotics therapy for a proper choice of initial antibiotics to improve outcome of the patients.
There are numerous infectious disease programs which provide both expert recommendations regarding antibiotic choice as well as background information (epidemiology, diagnostic studies, source of pathogens, etc).
The choice of empirical antimicrobial therapy depends on complex factors related to the underlying disease, susceptibility of pathogens, patient's history and clinical syndrome.
It shows that there is a discrepancy between the common MDR pathogen and the choice of empirical antibiotic therapy.
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