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To guide future expression strategies for new proteins, particularly regarding the choice of expressing a full-length protein in a bacterial or eukaryotic system and also where to truncate multi-domain containing proteins, it is interesting to investigate if the proteins expressed in a soluble form in this study share any common properties.
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Fig. 6 Proportion of choice in terms of expressing doubts about technology.
"As language users, we have a choice of many ways of expressing ourselves," Futrell says.
E. coli remains the system of first-choice for expressing proteins, as it is cheap and easy to handle, however many mammalian proteins cannot be successfully expressed in E. coli [ 4].
High-throughput cultivations in microtiter plates are the method of choice to express proteins from recombinant clone libraries.
The mammary gland has generally been considered the organ of choice to express valuable recombinant proteins in transgenic animal bioreactors because milk is easily collected in large volumes.
So the Congolese most in need of expressing their choice will be silent this election.
Israel's deputy foreign minister, Danny Ayalon, said: "If there was a shadow of hope for a change in Iran, the renewed choice of Ahmadinejad expresses more than anything the growing Iranian threat".
However, many biologists are uncertain about the choice of differentially expressed gene (DEG) analysis methods and the validity of cost-saving sample pooling strategies for their RNA-seq experiments.
In this study we exploited several advantages of using individual patient data: standardisation of outcome choice (pain-free walking distance), standardisation of expressing the outcome (geometric mean of relative improvement) across all studies, application of powerful analytical methods taking into account heterogeneity, and full intention to treat analysis.
Choice of gender expression, choice to love, choice to express and articulate my sexual desires.
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