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Despite normal olfactory ability, young adult male C58/J mice showed deficits in social approach in the three-chambered choice assay and failed to demonstrate social transmission of food preference.
This would have introduced considerable, uncontrolled variation in the parasitoid loads experienced by individual caterpillars during the feeding choice assay.
After the feeding choice assay, all caterpillars were reared on standard rearing food until they either pupated or died.
The paradigm of the choice assay we used [14] suggests that the observed repellency to DEET, IR3535, and picaridin (Fig. 1) is mediated by the fly's olfactory system.
FruM expression did not return to detectable levels after either the 10-minute courtship choice assay or the 1.5 h aggression assay (data not shown).
Using a previously described choice assay [14], we showed that the fruit fly is indeed repelled by DEET [14], [16], with no difference between male and female responses (Fig. 1A).
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A complementary group of experiments employed variations in a two-choice assay [15].
During the dual-choice assay, F. occidentalis individuals showed a marked preference for leaf discs from control plants over those from B. cinerea-infested plants (Fig. 4B).
During the dual-choice assay, R. porosum individuals showed a marked preference for leaf discs from control plants over those from B. cinerea-infested plants (Fig. 4A).
(B ) Oviposition-guidance preference for each odorant as assayed in the 3-choice assay.
In the two-choice assay, does vapor pressure of the molecule explain behavioral response thresholds and peak responses?
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