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The concentration of chloride was 2.8 M to simulate the concentration of chloride spread in many local regions of Saudi Arabia and called Sabkha.
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Fractographic and metallurgical studies indicate that the bolt failure is due to a deep crevice corrosion process that is further aggravated by the presence of chlorides coming from the spray formed inside tunnels during the winter when sodium chloride is spread for de-icing roads.
Then, mixture of the solutions of calcium chloride and urea was spread onto the sand surface and left for 16 h.
This spread between chlorides and sulfates is due to the mentioned difference in molecular weight between NaCl (58.44 g mol−1) and Na2SO4 (142.04 g mol−1).
As rapid spreading of ferric chloride solution allowed the study of only one or two arterioles and venules within each preparation, both left and right cremaster muscles of each animal were prepared for analysis of thrombotic vessel occlusion.
A droplet of sodium chloride solution placed on carbon steel spread over the surface forming tiny water micro-droplets and an expanded wet area without changing the droplet shape.
Thereafter, microvascular thrombosis was induced by spreading of 25 μl ferric chloride solution (12.5 mmol/l; Sigma) over the cremaster muscle every minute, resulting in a continuous superfusion of the tissue [ 11, 12].
Each subsample was plated, by both pour and spread plate techniques, onto tryptic soy agar 2% sodium chloride (TSAN2) with or without catalase (400 or 600 U) or sodium pyruvate (80 or 160 mg) supplementation.
Unilateral spreading cortical depression was elicited by applying potassium chloride solutions to the dura of conscious, freely moving rats.
To prevent the spread of diseases, UNICEF and the Ministry of Health also have provided chloride equipment, safe water, mattresses and insecticide-treated mosquito nets.
Inland sources of chloride include natural soil salts such as calcium chloride and de-icing compounds spread on winter roads.
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