Sentence examples for chloramine t solution from inspiring English sources

Prior to use, the teeth were stored in 0.5 % chloramine T solution for up to 7 days and subsequently in distilled water at 4 °C for a maximum of 6 months.

A volume (250 µL) of chloramine T solution was added to 500 µL of the sample and the mixture left for 20 min at room temperature.

The chloramine T solution and aldehyde-perchloric acid solution used for the assay were prepared as follows: Chloramine T (sodium N-chloro-p-toluenesulfonamide) 0.3535 g was dissolved in 5.175 mL of water, and then 6.5 mL of n-propanol and 13.325 mL of pH 6 buffer (citric acid monohydrate 25 g, acetic acid 6 mL, sodium acetate trihydrate 60 g, sodium hydroxide 17 g to 500 mL) were added.

Samples were treated with chloramine T solution and perchloric acid/aldehyde, as described by Stegemann and Stalder [ 24].

50 μl of each sample was reacted with 50 μl of chloramine T solution (Sigma Aldrich, Poole, UK) and allowed to oxidize at room temperature for 20 min.

One milliliter of potassium borate buffer (pH 8.7) was added to maintain constant pH and the sample was oxidized with 0.3 mL of chloramine T solution at room temperature for 20 minutes.

Hydrolyzed samples (20 μL) were added to 96-well plate and incubated for 20 min at room temperature with 50 μL/well of chloramines T solution (282 mg chloramines T, 2 mL n-propanol, 2 mL distilled water, and 16 mL citrate acetate buffer).

2. Solution A: One part 7% chloramine T-solution (Sigma) was mixed with 4 parts of citrate/acetate buffer (pH 6.0: 57 g sodium acetate.3H2O (Merck) + 37.5 g Na3citrate.2H2O (Sigma) + 5.03 g Citrate + 385 ml isopropanol made up to 1 liter with distilled water).

The teeth were stored for 7 days in a container with 0.5 % chloramine-T solution for disinfection.

Enamel and dentin specimens were obtained from fifty bovine incisors, which were properly cleaned, disinfected in 0.5% chloramine-T solution for seven days, and cut to remove the roots.

A chloramine-T solution (10 μl) at concentration of 2.5 mg ml−1 and NaI125 solution (40 μl) of total activity ~20 MBk were added to IgG (or BSA) solution (50 μl) at a protein concentration of 1 mg ml−1 in 0.5 M phosphate buffer (pH 7.0).