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Two Rabelaisian feasts, at which our group consumed prodigious amounts of sushi as the many-colored plates piled up like poker chips, were done in an hour.
ChIPs were done from at least four independent chromatin preparations, from which two were sent to NimbleGen.
RNA labeling and hybridization on miRNA microarray chips were done as described in detail elsewhere.
Hybridisation and washing of gene chips were done as previously described [ 16].
Target preparation, hybridization, washing, staining and scanning of the chips were done according to the manufacturer's protocol.
Hybridization and washing of gene chips were done in accordance with the manufacturer's instructions and were as described previously [ 13].
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Statistical analysis of individual animal per strain chips was done using the dChip software [ 11].
Hybridization of affymetrix chips was done following the standard Affymetrix protocol.
Washing and stripping of chips was done according to manufacturer's protocol.
Separation of CPs extracted from sweet potato chips was done by a DB-WAX column [ 37].
The scanning of the chips was done according to standard Affymetrix protocols.
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