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Avoid the exterior paint problems of cracking and chipped surfaces with better preparation, such as sanding, filling and priming exterior surfaces.
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Utilizing hydrophobicity interactions, the adhesion affinity between biomolecules and chip surfaces can be modulated by the applied electrical field.
The sensor-chip surfaces were regenerated with 100 mM alanine-HCl (pH 2.5).
Immobilized sensor chip surfaces were prepared using the Biacore amine Coupling Kit.
To regenerate chip surfaces, bound ligands were removed with 10 mM HCl.
Various concentrations of ADP, raloxifene, or 4-OHT analyte were injected over yeast Hsp82 coupled biosensor chip surfaces.
After this time, purified C4BP [11] was passed over the chip surfaces for 5 minutes at a concentration of 40 µg/ml, followed by 5-minute dissociation.
The QCM chip surfaces were analyzed by atomic force microscopy (AFM).
CM5 sensor chip surfaces were activated by using an amine coupling kit (GE Healthcare).
The analytes dissociated completely from the chip surfaces, eliminating the need for a regeneration step.
Furthermore, nanobodies can easily be coupled to beads, matrices, or even microarray chip surfaces, to generate antigen-specific nanotraps.
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