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For ChIP-qPCR, ChIP samples were diluted 1 20 and input samples 1 2000.
To validate this peak set, randomly selected targets were tested in ChIP-qPCR using three new biological ChIP samples (Figure 3B).
Immunoprecipitated DNA from ChIP samples was converted in sequencing libraries using ChIP-Seq DNA Sample Prep Kit (IP-102 1001). IP-102 1001
The re-ChIP DNA was eluted with ChIP elution buffer as described in the single ChIP protocol above, and ChIP samples were analyzed by qPCR.
Eventually Chip samples both the century-old whiskey and the new stuff.
NIR spectra were measured on the chip samples, and transformed to principal components.
This chapter provides protocols for ChIP, for quality control tests of ChIP samples, for microarray design, for hybridization of the ChIP samples onto microarrays, and for initial analysis of the data.
Rock chip samples (n = 14) were also collected from a 24 m deep core drilled into the northern ridge top.
ChIP samples were quantitated by real-time PCR.
ChIP samples were obtained using K562 cells, amplified, and applied to the genomic tiling arrays.
Three biological replicas were performed for the MNase digested ChIP samples.
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