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But the chip figures hugely here.
Primers for ChIP SYBR green primer sets were used for all ChIP figures.
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Figure 5 presents the SPR sensorgrams BI-3000GG systemstem) of a Au-MOA film (conventional SPR chip) (Figure 5a), a Au-Cys-GOS film (GOS film-based SPR chip) (Figure 5b), and a Au-ODT-GOS film (ODT-based GOS film-based SPR chip) (Figure 5c), in response to solutions of BSA with a concentration of 100 μg/ml in phosphate buffered saline (PBS) buffer.
Binding of Oct4 to several predicted PORE targets was verified via chromatin immunoprecipitation (ChIP, Figure 3B).
As expected, the number of peaks called decreases in simulations of less efficient ChIP (Figure S6).
To do this, we synthesized a DNA array with a cleavable linker on the 3' end (closest to the chip) (Figure 6).
The custom designed biphasic electrical current stimulator chip (Figure 1) allowed easy access to control the duration and amplitude of electric currents being applied to the NSCs.
Functionalized nanotubes are captured in the microchannel by application followed by applying an electric field (1 MHz and 1V) to the microelectrode chip (Figure 1) [13].
The RNA primers used to detect the membrane bound, long form of the IL1R2 message were derived from the coding region of the IL1R2 gene present on the Affymetrix gene array chip (figure 2A).
Under all of these conditions, changes in H3K4me3 and WRKY70 transcripts correlated with the presence of ATX1 at the promoter nucleosomes, as assessed by ChIP (Figure 5c), suggesting that PtdIns5P may regulate ATX1 localization.
When a mixture of sCD154 and C4BP was flowed over the chip (figure 2 lower panel) binding was achieved confirming in agreement with Brodeur and collegues, that the presence of C4BP did not prevent sCD154 binding to CD40 (see below).
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