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Animals were housed 2 3 per cage (same age cage mates) in clear polycarbonate cages with wood chip bedding.
The animals were housed in a pathogen free colony on hardwood chip bedding in microisolator cages at the University of Tennessee Health Science Center, Memphis, TN.
Mice were housed on hardwood chip bedding in open-top cages.
Animals were housed in a pathogen-free isolation barrier facility with chip bedding.
The rats were housed in polycarbonate cages with hardwood chip bedding.
Animals were housed in sterilized cages (5 mice/cage) with hardwood chip bedding.
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The testing apparatus consisted of a plastic arena (45-cm long, 15-cm high, and 30 cm wide) with the floor covered by 2 cm of wood-chip bedding.
Mice are housed in clear polycarbonate cages (Makrolon type II cage, 22 cm × 16 cm × 14 cm) with wood-chip bedding covered floors.
A total of 30 four-week-old male Sprague Dawley rats (Rattus Norwegicus), weighing approximately 90 100 grams were housed in plastic cages (two rats per cage) with wood-chip bedding.
The cleaning routine was standard across both separated and non-separated rats with approximately half of the soiled wood-chip bedding removed every third day in the first week and every second day in the second and third weeks following birth.
Twenty-four hours later, observers are then assigned to demonstrators (1 demonstrator assigned for 2 observers, counterbalanced by observer group) and are habituated to social interaction by being placed in a social interaction arena (23.5 cm W × 44.45 cm L × 27 cm H; clear plastic bin with Sani-chip bedding covering the bottom) to interact with each other for 30 min.
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