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ChIP assay was performed using the ChIP Assay Kit from Millipore (Billerica, MA, USA), following the supplied protocol.
We performed the ChIP assay according to the instructions of the ChIP assay kit (Upstate, USA).
The ChIP assay was performed according to the protocol of ChIP assay kit (Millipore).
Cells were harvested and CHIP assays were processed using the Chromatin Immunoprecipitation (CHIP) Assay Kit (Upstate).
GCs from three rats were pooled at each time point to use for ChIP assay.
A ChIP assay was performed using the Pierce Agarose ChIP kit as described previously23.
ChIP assay was performed with antibodies to EZH2 and control IgG.
Each ChIP assay was validated by qPCR against an IgG control and enrichment above input.
ChIP assay was used to detect the chromatin environment of TET1 promoter.
Quantitative ChIP assay was performed as previously described70 with some modification.
ChIP assay was performed with antibodies to C/EBPβ, HIF1α and control IgG.
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