One has only to recall Mr. Louis in his 1966 solo, "Chimera," to realize his flair for the fantastic.
By further engineering the transmembrane domain of the chimera to reduce interchain interaction we attained stricter ligand-dependency.
Second, we localized the Ca2+-activated RhoA chimera to the plasma membrane, where it responded specifically to local Ca2+ signals.
To this end, a 20-kDa polyethylene glycol (PEG) was attached to the chimera to increase circulatory half-life of the chimera in mice (Supp. Fig. 1 27.
He sought to attain a timelessness that he sensed in early composers like Monteverdi, and fabricated his own medieval chimera to that end.
To optimize gene expression in breast cancer cells in vitro and in vivo, we created a new promoter enhancer chimera to regulate gene expression.
They replicated the chimera-mediated mechanosensitive currents in HEK293T cells that have nearly no detectable expression of endogenous Piezo1, but paradoxically found the chimera to be less effective in Piezo1 knockout HEK293T cells, indicating the involvement of endogenous Piezo1.
Lots of musicians of that period – from the crazy art-punk group Chimera to the psycho-bard Venya Drkin – never really took off largely because they didn't fit the infamous "radio format"... Whereas a tonne of glam hacks got all the glory.
However, when this domain was joined with the extracellular and transmembrane domains of the epidermal growth factor receptor, it was not sufficient to direct this chimera to late endosomes and the trans Golgi network (Dintzis, S. M., and Pfeffer, S. R. (1990) EMBO J. 9, 77-84).
While giving the impression that it had created variants to target certain types of pain, in reality it made a marketing chimera to target vulnerable people, knowingly persuading sufferers to buy duplicate medicines in the false belief that they were aimed at different ailments.
