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In this Matters Arising Response, we discuss the chimera results and consider potential interpretations in light of the Matters Arising from Dubin et al.
This approach simplified the interpretation of our ORF2 chimera results, since an additional complicating variable (human or mouse ORF1) was not required to assess functionality.
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Treatment with EphB4/Fc chimera resulted in activation of PI3K, Src, and MAPK pathways.
Rac1CTCdc42 chimera resulted from the replacement of the region encoding the last carboxyl-terminal 14 residues of RAC1 by that of the last carboxyl-terminal 12 of CDC42.
The reciprocal Cdc42CTRac1 chimera resulted from the replacement of the region encoding the last carboxyl-terminal 12 residues of CDC42 by that of the last carboxyl-terminal 14 of RAC1.
HsRac1CTCaRac1 chimera resulted from the replacement of the region encoding the last carboxyl-terminal 12 residues of HsRAC1 by that of the last carboxyl-terminal 14 residues of CaRAC1.
A similar inhibitory phenotype was evident for the Y543F mutant, but BCR co-ligation with the Y556F chimera resulted in effects analogous to the WT.
Surprisingly, removal of phosphorylation sites from this chimera resulted in a receptor that is constitutively internalized, even in the absence of agonist.
We did not detect partial homology with other rdhA that would suggest vcrA is a chimera resulting from a recent homologous recombination event.
Overexpression of the mChFP-Tmod2/Tmod1 chimera resulted in a 1.5-fold decrease in the number of neurites (2.2 ± 0.98 neurites/cell) compared to the number seen with GFP-Tmod1, 3.1 ± 1.22 neurites/cell.
A real-life example that illustrates the possibility that chimera research might disrupt a useful social practice is the attempt in 1998 by Stuart Newman (a professor of cell biology and anatomy) and Jeremy Rifkin (an anti-biotechnology activist) to patent a broad range of chimeras resulting from the mixing of human and nonhuman embryonic cells (Heathcotte and Robert 2006; Rabin 2006).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com