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This study demonstrates that Rhesus macaques immunized with a DNA plasmid vaccine-encoding gag as an hLAMP/gag chimera develops strong antigen-specific humoral responses as well as CD4+ and CD8+ T-cell responses.
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Here, all but one chimera developed mesothelioma with thoracic Luciferase expression; however, the increase in Luciferase expression over time was limited and in some cases leveled off after an initial increase (supplementary Fig S6).
As a case study, we apply our model to different mutants of a IFNα-2a-EGF chimera developed in ref. 14, in which the EGF subunit targets cells overexpressing EGFR and the IFN subunit triggers apoptotic signals.
Moreover, DC-LMP1/CD40 chimeras developed inflammatory bowel disease characterized by massive transmural influx of leukocytes and lymphocytes, resulting in villous degeneration and lipid malabsorption.
The utrophin mutation in mdx∶utrophin chimeras appeared to affect the rescue process by the iPSCs, as the double mutant chimeras developed an abnormal fibrotic musculature.
The u-PA-/ → C57 chimeras developed disease similar to u-PA-/ mice; 56% (seven out of 11) of the chimeras developed arthritis with a mean maximum clinical score of 1.2 ± 0.4.
Human-animal chimeras, developed with a 'humanized' immune system could be useful to study infectious diseases, including many neglected diseases.
The Bid chimeras developed in this study are targeted to membranes to better represent the interactions between BH3-only proteins and Bak and Bax that require a membrane.
Only three of the control chimeras developed symptoms and no animals in this cohort died during the 8-month-long follow-up period (Fig. 4B).
All NSCLC chimeras developed multiple lesions in the lung ranging from adenomatous or bronchioalveolar hyperplasia to bronchioalveolar adenomas, adenocarcinomas and papillary carcinomas (supplementary Fig S2B and C).
When HD was expressed only in vegetal halves, the resulting chimeras developed into normal plutei (n = 10/10), which endomesodermal territories appeared red-fluorescent.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com